Nhs Activation Of Sepharose 4
NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix. That increases the choice of coupling chemistries available. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with. Ligands containing primary amino groups. All FAC data were obtained in 10 mM Tris-Cl buffer, pH 7.4, containing 0.7% NaCl (TBS) at a flow rate of 0.125 mL/min with the FAC-1 machine, which had been specially designed and manufactured by Shimadzu Purified AH (1.0 mg) was immobilized on 100 μL of NHS-activated Sepharose resin (Amersham Bioscience).
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NHS-activated Sepharose 4 Fast Flow is a pre-activated agarose matrix that increases the choice of coupling chemistries available. Suitable for coupling amino-containing smaller proteins and peptides. • NHS pre-activated resin for coupling of small amino-containing proteins and peptides in process-scale applications • High level of pre-activation gives a high degree of substitution of the selected ligand • NHS coupling method results in chemically stable ligand attachment • BioProcess resin supported for industrial applications.
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